Phew it's been a while! I've been busy, but in all honesty I haven't done a Transcriptic blog recently because I've been doing a lot of repetition trying to optimise plating bacteria. There's a lot to write about, but I think it would be best for both you and I if I try and keep it short. In this post I want to cover doing some rough analysis of early data from my burden assay project on Transcriptic.
Flow chemistry is pretty hot right now, check out anything by Steve Ley he does some cool stuff. Flow chemistry and microfluidics are really popular, they enable precision control over the spatial distribution of materials during continuous processes. This is useful when one wants to continuously combine materials such as in a chemical process ultimately yielding a higher throughput when compared to batch processing. Flow chemistry and microfluidics also enable the emulation of biological systems, replicating similar kinetics and shear flows at surfaces which are not necessarily possible in bulk processes.
On my first attempt at translating an experimental protocol to the Autoprotocol format I got as far as creating a run on Transcriptic with the protocol which is awesome. The downside of that Autoprotocol though, was that the samples being used in the experiment were hardcoded into the python script, so any variation in experimental samples or parameters would have to be made in the python.